Description
Intended Use The C-reactive protein assay is intended for the detection of human C-reactive protein (CRP) in human serum.
Principles of the Test Human sera for testing are diluted to 1:4,000 and allowed to react with antibodies coated on specially treated micro-wells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzyme-labeled rabbit anti-human CRP (conjugate) is then added to react with and tag the antigen-antibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450nm on a spectrophotometer or ELISA reader. Assigning a value for the absorbance can be accomplished by the use of a standard curve generated by measuring two-fold dilutions of the standard provided